Polyol Conversion Specificity ofBacillus pallidus

Glycerol dehydrogenase. structure, specificity, and mechanism of a family III polyol dehydrogenase.

BACKGROUND Bacillus stearothermophilus glycerol dehydrogenase (GlyDH) (glycerol:NAD(+) 2-oxidoreductase, EC 1.1.1.6) catalyzes the oxidation of glycerol to dihydroxyacetone (1,3-dihydroxypropanone) with concomitant reduction of NAD(+) to NADH. Analysis of the sequence of this enzyme indicates that it is a member of the so-called iron-containing alcohol dehydrogenase family. Despite this sequenc...

Polyol specificity of recombinant Arabidopsis thaliana sorbitol dehydrogenase studied by enzyme kinetics and in silico modeling

Polyols are enzymatically-produced plant compounds which can act as compatible solutes during periods of abiotic stress. Nicotinamide adenine dinucleotide(+)-dependent SORBITOL DEHYDROGENASE (SDH, E. C. 1.1.1.14) from Arabidopsis thaliana L. sorbitol dehydrogenase (AtSDH) is capable of oxidizing several polyols including sorbitol, ribitol, and xylitol. In the present study, enzymatic assays usi...

Evaluating and optimizing pretreatment technique for catalytic hydrogenolysis conversion of corn stalk into polyol.

A combinative pretreatment technology of steam explosion (SE) and alkali was applied to enhance hydrogenolysis conversion of corn stalk into polyol with Ni-W2C or Fe-Mn-K catalyst. The results showed that treatments corn stalk with 0.4 MPa SE and alkali removed 84.16 wt% of hemicellulose and 71.83 wt% of lignin and thereby increased the cellulose content from 31.54 to 80.41 wt%. But the glucose...

Polyol Laxative Threshold Value

Sequence of the N-Terminal Half ofBacillus amyloliquefaciens a-Amylase

Bacillus amyloliquefaciens a-amylase (1,4-a-D-glucan glucanohydrolase. EC 3.2.1.1), which is commercially supplied as 'Bacillus subtilis a-amylase' does not cross-react immunologically with B. subtilis a-amylase. This enzyme (from B. amyloliquefaciens) was cleaved by treatment with CNBr into seven fragments. Peptide A was selected for sequence determination. It is the longest one, containing 18...